| Seiyuu | Role |
| Tomoko Kawakami | Rosette Christopher |
| Akira Ishida | Chrno |
| Tomomichi Nishimura | Elder (Edward Hamilton) |
| Michiko Neya | Satella |
| Kazuhiko Inoue | Ion |
| Natsuko Kuwatani | Fiore |
| Junko Minagawa | Joshua |
| Shou Hayami | Remington |
| Yoshiko Sakakibara | Kate Valentine |
| Saeko Chiba | Asmaria Hendrick |
| Noriko Rikimaru | Sister Claire |
| Eiji Maruyama | Steiner (Episode 6, 12, 14 - 19) |
| Hiroshi Matsumoto | Gilliam (Episode 15 - 22) |
| Ema Kogure | Sister Mary |
| Yuki Masuda | Sister Anna |
| Kouichi Tohchika | Jenai (Episode 15 - 18, 20) |
| Yoko Soumi | Rizel (Episode 8, 13, 15, 21) |
| Norio Wakamoto | Dufaux (Episode 14 - 21) |
| Yuuko Sasamoto | Sheda (Episode 15, 17 - 21) |
| Ema Kogure | Nelly (Episode 9 & 10) |
| Yuki Masuda | Kevin (Episode 9 & 10) |
| Tomoko Kawakami | Magdalena (Episode 21) |
| Iemasa Kayumi | Ricardo Hendrick (Episode 3 & 4) |
| Hisao Egawa | Viscount Bolzo (Episode 2) |
| Hiroshi Yanaka | Viscount Relaie (Episode 3 & 4) |
| Yumi Takada | Elisa (Episode 16) |