| Seiyuu | Role |
| Noriaki Sugiyama | Shirou Emiya |
| Ayako Kawasumi | Saber |
| Kana Ueda | Rin Toosaka |
| Junichi Suwabe | Archer |
| Noriko Shitaya | Sakura Matou |
| Miki Itou | Fujimura-sensei |
| Jouji Nakata | Kirei Kotomine |
| Mai Kadowaki | Illya |
| Mitsuaki Madono | Issei Ryoudou |
| Kazuhiro Nakata | Souichirou Kuzuki |
| Yuu Asakawa | Rider |
| Nobutoshi Hayashi | Lancer |
| Atsuko Tanaka | Caster |
| Hiroshi Kamiya | Shinji Matou |
| Tadahisa Saizen | Berserker |
| Shinichirou Miki | Asashin (Episode 8 - 10, 18 - 20) |
| Tomokazu Seki | Gilgamesh (Episode 19 - 24) |
| Rikiya Koyama | Kiritsugu Emiya (Shirou's Father) |
| Fumie Mizusawa | Ayako Mitsuzuri (Episode 1 & 2) |
| Junko Noda | Shirou (as a child Episode 1 & 4) |
| Kouji Tsujitani | Rin's Father (Episode 2) |
| Houko Kuwashima | Mordret (Episode 21) |
| Mamiko Noto | Bediviere (Episode 24) |
| Michiru Yuimoto | Kaede Makidera (Episode 24) |