| Seiyuu | Role |
| Romi Paku | Edward Elric |
| Rie Kugimiya | Alphonse Elric |
| Toru Ohkawa | Roy Mustang |
| Kenji Utsumi | Alex Louie Armstrong |
| Megumi Toyoguchi | Winry Rockbell |
| Keiji Fujiwara | Maes Hughes |
| Yuko Satou | Lust |
| Ryoutarou Okiayu | Scar |
| Houko Kuwashima | Roze |
| Kotono Mitsuishi | Glacier Hughes |
| Miyoko Asoh | Pinaco Rockbell (Winry's Grandmother) |
| Michiko Neya | Lisa Hawkeye |
| Jin Horikawa | General Hakuro |
| Yasuhiro Takato | Gluttony |
| Yumi Kakazu | Lyla |
| Yasunori Matsumoto | John Havoc |
| Kentarou Itou | Barry the Chopper (Episode 8, 19 - 21, 24) |
| Tomoyuki Shimura | Heymans Breda |
| Tetsu Shiratori | Kain Furry |
| Mayumi Yamaguchi | Envy |
| Yuuji Ueda | Kimbly |
| Mitsuki Saiga | Maria Ross |
| Junichi Suwabe | Greed (Episode 21, 30 - 34) |
| Mayumi Yoshida | Elicia Hughes |
| Eriko Kawasaki | Rick (Episode 18, 24, 25, 32, 36) |
| Jun Fukushima | Leo (Episode 24, 25, 32, 36) |
| Naomi Wakabayashi | Sheska |
| Keiichi Sonobe | Mason (Episode 27 - 32) |
| Shou Hayami | Frank Archer |
| Yoshino Takamori | Sloth |
| Hidekatsu Shibata | King Bradley |
| Nana Mizuki | Wrath |
| Rumi Kasahara | Martel |
| Shouko Tsuda | Izumi |
| Seiji Sasaki | Sig (Izumi's Husband) |
| Kazuko Sugiyama | Dante (Episode 32, 33, 46, 47) |
| Takehiro Murozono | Farman |
| Masashi Ebara | Hohenheim (Episode 43 - 45, 49 - 51) |
| Akiko Yajima | Klose (Episode 4) |
| Satomi Koorogi | Nina Tucker (Episode 6 & 7) |
| Takaya Hashi | Majihal (Episode 4) |
| Akimitsu Takase | Clay (Episode 1, 2, 14) |
| Yoshino Takamori | Edward's Mother (Episode 2, 3, 10) |
| Kouji Ishii | Bald (Episode 5) |
| Toshihiko Seki | Vercio (Episode 11 & 12) |
| Motoko Kumai | Elisa (Episode 11 & 12) |
| Kazuki Yao | Yoki (Episode 9, 30, 32) |
| Tomoko Kawakami | Kayal (Episode 9) |
| Jouji Nakata | Holling (Episode 9) |
| Fumihiko Tachiki | Police Detective (Episode 10) |
| Hideyuki Tanaka | Nash (Episode 12) |
| Kinryu Arimoto | Conero (Episode 1, 2, 14) |
| Minako Arakawa | Fletcher Tringham (Episode 11, 12, 48, 49) |
| Kyoko Hikami | Paninja (Episode 26) |
| Kousuke Okano | Russel Tringham (Episode 11, 12, 48, 49) |
| Daisuke Gouri | Dominique (Episode 26) |
| Takehito Koyasu | Lujon (Episode 35) |
| Fumiko Orikasa | Livia (Episode 35) |
| Eiji Maruyama | Ishbal Camp Eldest (Episode 18, 24, 25) |
| Aya Ishizu | Barry the Chopper (female Episode 8) |
| Makoto Tsumura | Selim (Episode 49 - 51) |
| Yuriko Yamaguchi | Winry's Mother (Episode 3) |
| Emi Motoi | Marin (Episode 5) |
| Keiichi Sonobe | Klose's Father (Episode 4) |
| Rumi Ochiai | Marin's Mother (Episode 5) |
| Yuko Kaida | Rick's Mother (Episode 24) |
| Rie Kugimiya | Cathrine Armstrong (Episode 37) |
| Romi Paku | Alex's Mother (Episode 37) |
| Mitsuaki Madono | Kain (Episode 2) |
| Takehiro Murozono | Winry's Father (Episode 3) |
| Kozue Komiya | Levi (Episode 4) |