| Seiyuu | Role |
| Jouji Nakata | Alucard |
| Fumiko Orikasa | Ceras Victoria |
| Unsho Ishizuka | Peter Ferguson |
| Yoshiko Sakakibara | Integra Wingates |
| Takumi Yamazaki | Incognito (Episode 9 - 13) |
| Motomu Kiyokawa | Walter |
| Wataru Takagi | Leif (Episode 2) |
| Ryoutarou Okiayu | Enrico (Episode 3) |
| Yuuji Takada | Chris Pickman (Episode 9, 11, 12) |
| Katsuhiro Kitagawa | Sir Penwood (Episode 5 & 6) |
| Ryuji Mizuno | Sir Islands (Episode 5 - 7) |
| Masahiko Tanaka | Clergyman (Episode 1) |
| Jin Horikawa | Sir Peter Winfield (Episode 1) |
| Katsuyuki Konishi | Mason (Episode 7) |
| Yasunori Matsumoto | Boz (Episode 4) |
| Kaori Mizuhashi | Integra (as a child Episode 10) |
| Kazuya Nakai | Jan Valentine (Episode 5 & 6) |
| Chikao Ohtsuka | Sir Hellsing (Episode 10) |
| Hideyuki Tanaka | Maxwell (Episode 7) |
| Takehito Koyasu | Luke Valentine (Episode 5 & 6) |
| Akeno Watanabe | Jessica (Episode 2) |
| Norio Wakamoto | Richard (Episode 10) |
| Akiko Hiramatsu | Helena (Episode 8 & 11) |
| Ken Narita | Paul Wilson (Episode 9, 11, 12) |
| Mika Doi | Laura (Episode 9) |
| Hideyuki Umezu | Steadler (Episode 4) |
| Tomoyuki Shimura | Gareth (Episode 2 & 3) |
| Jin Yamanoi | Jack (Episode 1) |