| Seiyuu | Role |
| Yuuji Ueda | Keitarou Urashima |
| Yui Horie | Naru Narusegawa |
| Megumi Hayashibara | Haruka Urashima |
| Satsuki Yukino | Mutsumi Otohime |
| Junko Noda | Mitsune Konno |
| Yuu Asakawa | Motoko Aoyama |
| Reiko Takagi | Kaolla Suu |
| Masayo Kurata | Shinobu Maehara |
| Shouzou Iizuka | Daimaou (Episode 8) |
| Satomi Koorogi | Moe (Episode 20 & Love Hina Again) |
| Yuki Matsuoka | Kuro (Love Hina Again) |
| Natsuko Kuwatani | Kanako Urashima (Love Hina Again) |
| Hiromi Tsuru | Shinobu's Mother |
| Ryoutarou Okiayu | Kentarou Sakata |
| Yasunori Matsumoto | Noriyasu Seta |
| Masashi Ebara | Shinobu's Father |
| Nana Mizuki | Nyamo (Spring Special) |
| Aya Hisakawa | Amara Suu |
| Yuri Shiratori | Mei Narusegawa |
| Hiroyuki Yoshino | Masayuki Haitani |
| Yumiko Kobayashi | Sarah MacDougal |
| Miki Nagasawa | Tsuruko Aoyama (Episode 25) |