| Seiyuu | Role |
| Sanae Kobayashi | Madlax |
| Houko Kuwashima | Margaret Burton |
| Satsuki Yukino | Vanessa |
| Toshiyuki Morikawa | Carossur |
| Tomoko Kaneda | Letitia |
| Ai Uchikawa | Elinor |
| Aya Hisakawa | Limerda |
| Masashi Ebara | Friday |
| Mako Hyoudou | Kuanjitta |
| Tokuyoshi Kawashima | Charlie |
| Daisuke Namikawa | Pete (Episode 1) |
| Kaori Nazuka | Annu (Episode 4) |
| Miyu Irino | Chris (Episode 5, 16, 21) |
| Saeko Chiba | Chiara (Episode 6) |
| Akira Ishida | Lopez (Episode 6) |
| Shigenori Souya | Feric (Episode 6) |
| Emi Shinohara | Lucille (Episode 7 & 8) |
| Hiroaki Hirata | Eric (Episode 7, 8, 16, 19) |
| Kinryu Arimoto | Gwen McNicol (Episode 3 & 16) |
| Masaki Terasoma | Macrae (Episode 4) |
| Nozomu Sasaki | Budgis (Episode 10 - 12, 17) |
| Jun Fukuyama | Eins (Episode 11) |
| Toru Ohkawa | Margeret's Father (Episode 21 & 25) |
| Kotono Mitsuishi | Margaret's Mother (Episode 21 & 25) |
| Toru Ohkawa | Margaret's Math Teacher |