| Seiyuu | Role |
| Mayo Suzukaze | Kenshin Himura |
| Miina Tominaga | Yahiko Myoujin |
| Yuuji Ueda | Sanosuke Sagara |
| Kazuhiko Inoue | Takimi Shigure |
| Yuko Miyamura | Toki Takatsuki |
| Takehito Koyasu | Kajiki |
| Kappei Yamaguchi | Musashino |
| Hiroshi Yanaka | Tsunan Tsukioka |
| Juurouta Kosugi | Eibin Tamono |
| Nozomu Sasaki | Gentatsu Takatsuki |
| Hirotaka Suzuoki | Hajime Saitou |
| Noriko Namiki | Suzume |