| Seiyuu | Role |
| Katsuyuki Konishi | Kyo / Kyoushirou Mibu |
| Yui Horie | Yuya Shiina |
| Megumi Ogata | Yukimura Sanada |
| Akira Ishida | Sasuke Sarutobi |
| Yumi Kakazu | Okuni Izumono |
| Toshihiko Seki | Benitora |
| Yukana Nogami | Sakuya |
| Souichirou Hoshi | Akira |
| Shou Hayami | Nobunaga Oda |
| Hikaru Midorikawa | Migeira |
| Takashi Matsuyama | Saizou |
| Megumi Ogata | Kosuke Anayama |
| Miki Machii | Santera (Episode 4, 8, 14, 15, 17, 18) |
| Takehito Koyasu | Hanzou Hattori |
| Mikako Takahashi | Mahiro (Episode 4 - 6, 9, 18 - 26) |
| Akio Suyama | Makora |
| Takayuki Yamaguchi | Shindara |
| Norihisa Mori | Basara (Episode 10, 12 - 17) |
| Kazuhiko Inoue | Muramasa (Episode 19 - 21) |
| Takehito Koyasu | Hotaru (Episode 20 - 24, 26) |
| Sakiko Tamagawa | Shatora (Episode 13 - 18, 21 - 26) |
| Tomokazu Seki | Shinrei (Episode 19 - 26) |
| Takahiro Sakurai | Kubira (Episode 10 & 17) |
| Shinpachi Tsuji | Ieyasu Tokugawa (1, 4, 6 - 8) |
| Nana Mizuki | Mika (Episode 2 & 3) |
| Kouji Ishii | Mika's Father (Episode 2, 3, 17) |
| Kouki Miyata | Shirogarasu (Episode 2 & 3) |
| Hajime Iijima | Genma Kitou (Episode 2 & 3) |
| Hiroshi Iida | Kurosasori (Episode 3 & 17) |
| Keiichi Sonobe | Tadaaki Ono (Episode 6 - 9) |
| Susumu Chiba | Nobuyuki Sanada (Episode 8, 18, 26) |
| Kana Ueda | Antera (Episode 10, 13, 17) |
| Katsuhiro Harasawa | Bikara (Episode 10, 13, 17) |
| Yumiko Kobayashi | Sasuke (as a child Episode 13 & 24) |
| Kana Ueda | Saisei (Episode 18, 21, 22) |
| Sakura Nogawa | Saishi (Episode 18, 21, 22) |
| Hiroki Takahashi | Katsuie Shibata (Episode 25) |
| Asami Imai | Kotarou / Makora (as a child Episode 13 & 24) |
| Makoto Yasumura | Mekira (Episode 11 & 17) |