| Seiyuu | Role |
| Masaya Onosaka | Vash the Stampede |
| Shou Hayami | Nicholas D. Wolfwood |
| Toshihiko Seki | Legato Bluesummers |
| Satsuki Yukino | Milly Thompson |
| Hiromi Tsuru | Meryl Stryfe |
| Yoko Soumi | Dominique the Cyclopse (Episode 15) |
| Furin Cha | Nagi (Episode 15) |
| Hidetoshi Nakamura | Morgan (Episode 14) |
| Akio Ohtsuka | Rai Dei the Blade (Episode 16) |
| Juurouta Kosugi | Joey (Episode 17) |
| Tomohiro Nishimura | Midvalley the Hornfreak (Episode 15, 16, 24) |
| Nobuo Tobita | Patricia (Episode 14) |
| Miki Itou | Marilyn (Episode 14) |
| Kiyoyuki Yanada | Badwick (Episode 14) |
| Toru Furusawa | Millions Knives (Episode 12, 18, 24 - 26) |
| Yuki Masuda | Moore (Episode 11) |
| Maya Okamoto | Mary (Episode 17) |
| Kyoko Tsuruno | Lina (Episode 18) |
| Yuko Kobayashi | Karen (Episode 19) |
| Kyousei Tsukui | Steve (Episode 17) |
| Yukimasa Kishino | Ruth Loose (Episode 1) |
| Hisao Egawa | Marvin (Episode 4) |
| Hirotaka Suzuoki | Chapel the Evergreen (Episode 23 & 24) |
| Kenji Nojima | Richie (Episode 22) |
| Hiroshi Kamiya | Zazie the Beast (Episode 22) |
| Keiichi Nanba | Hoppered the Gauntlet (Episode 21) |
| Emi Motoi | Jessica (Episode 20 - 22) |
| Kouji Ishii | Brad (Episode 20 & 21) |
| Hiroshi Yanaka | Mark (Episode 19) |
| Tetsuya Iwanaga | Julius (Episode 11) |
| Miki Nagasawa | Michelle (Episode 10) |
| Atsuko Tanaka | Claire (Episode 10) |
| Kyoko Hikami | Stephanie (Episode 4) |
| Hidenari Ugaki | Simon (Episode 25) |
| Hidenari Ugaki | Mohican (Episode 18) |
| Rei Sakuma | Marianne (Episode 2) |
| Daisuke Gouri | Descartes (Episode 1) |
| Shigeru Nakahara | Rowan (Episode 17) |
| Takashi Nagasako | Kite's Father (Episode 8) |
| Masahiko Tanaka | Frank Marlon (Episode 3) |
| Kouki Miyata | Vash the Stampede (as a child) |
| Rika Matsumoto | Kite (Episode 6 - 8) |
| Aya Hisakawa | Rem |
| Takeshi Aono | Stan (Episode 4) |
| Ryuzaburo Ohtomo | Denim (Episode 4) |
| Mika Kanai | Neil (Episode 10) |
| Yukari Tamura | Helen (Episode 9) |
| Unsho Ishizuka | B.D. Neon (Episode 7 & 8) |
| Toshiyuki Morikawa | Keskass (Episode 6) |
| Yuri Amano | Elizabeth (Episode 6) |
| Yoshiko Kamei | Tonis's Mother (Episode 5 & 6) |
| Wakana Yamazaki | Monica (Episode 5) |
| Norio Wakamoto | Gofsef (Episode 5) |
| Kenichi Ogata | Professor Nebraska (Episode 5) |
| Kenji Nomura | Tills (Episode 25) |
| Isshin Chiba | Gene (Episode 4) |
| Masashi Sugawara | Ingway (Episode 4) |