| Seiyuu | Role |
| Kenichi Suzumura | Shiki Tohno |
| Hitomi Nabatame | Arcueid |
| Yumi Kakazu | Hisui |
| Fumiko Orikasa | Ciel |
| Shizuka Itou | Akiha Tohno |
| Kana Ueda | Kohaku |
| Takahiro Sakurai | Arihiko Inui |
| Kaori Tanaka | Satsuki Yumizuka |
| Akiko Kimura | Aoko Aozaki (Episode 1 - 3, 10 - 12) |
| Hiroyuki Yoshino | Roa (Episode 8, 9, 11, 12) |
| Keiko Nemoto | Shiki (as a child) |
| Kenta Miyake | Nero (Episode 2 & 3) |
| Takako Honda | Shiki's Mother (Episode 9) |
| Takako Honda | Roa (as a child Episode 11) |
| Kinryu Arimoto | Akiha's Father (Episode 11) |